IFIH1 Knockout cell line (THP-1)

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IFIH1 Knockout cell line (THP-1) Order

IFIH1 Knockout cell line (THP-1)
Catalog#
YKO-HT0241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IFIH1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IFIH1 Knockout cell line (THP-1)
Species
Human
Gene Name
IFIH1
Gene ID
64135
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript IFIH1-206)
Validation: PCR+Sanger Sequencing
IFIH1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IFIH1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IFIH1 gene knockout IFIH1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IFIH1 Knockout cell line (THP-1)
IFIH1 Knockout cell line (THP-1)
Catalog#
YKO-HT0241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • Ifih1 Knockout cell line (Raw 264.7)

    Ifih1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Ifih1
    Gene ID: 71586
    Size: 1*10^6
    Catalog#: YKO-MT0083
    YKO-MT0083(Raw 264.7)
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
    YKO-H825(293T)
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312
    YKO-HS14312(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Ifih1 Knockout cell line (Raw 264.7)

    Ifih1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Ifih1
    Gene ID: 71586
    Size: 1*10^6
    Catalog#: YKO-MT0083
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312

IFIH1 Knockout cell line (THP-1) Order

IFIH1 Knockout cell line (THP-1)
Catalog#
YKO-HT0241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IFIH1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IFIH1 Knockout cell line (THP-1)
Species
Human
Gene Name
IFIH1
Gene ID
64135
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript IFIH1-206)
Validation: PCR+Sanger Sequencing
IFIH1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IFIH1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IFIH1 gene knockout IFIH1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IFIH1 Knockout cell line (THP-1)
IFIH1 Knockout cell line (THP-1)
Catalog#
YKO-HT0241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Ifih1 Knockout cell line (Raw 264.7)

    Ifih1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Ifih1
    Gene ID: 71586
    Size: 1*10^6
    Catalog#: YKO-MT0083
    YKO-MT0083(Raw 264.7)
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
    YKO-H825(293T)
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312
    YKO-HS14312(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Ifih1 Knockout cell line (Raw 264.7)

    Ifih1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Ifih1
    Gene ID: 71586
    Size: 1*10^6
    Catalog#: YKO-MT0083
  • IFIH1 Knockout cell line (293T)

    IFIH1 Knockout cell line (293T)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-H825
  • IFIH1 Knockout cell line (HeLa)

    IFIH1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IFIH1
    Gene ID: 64135
    Size: 1*10^6
    Catalog#: YKO-HS14312

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